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| Registros recuperados : 42 | |
| 2. |  | CENTENO, D. da C.; MACHADO, D. N.; SILVA, M. A. P.; LOUREIRO, M. E. Atividades da proteína desacopladora e da hexocinase em mitocôndrias de tubérculos de batata (Solanum tuberosum L.) In: CONGRESSO NACIONAL DE BOTÂNICA, 55.; ENCONTRO REGIONAL DE BOTÂNICOS DE MG, BA, ES, 26., 2004, Viçosa. Conservação bioprospeccção e biotecnologia: [livro de resumos]. Viçosa: Sociedade Botânica do Brasil: Universidade Federal de Viçosa, 2004. CD-ROM.| Biblioteca(s): Embrapa Hortaliças. |
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| 5. | | CARVALHO, L. J. C. B.; CASCARDO, J. C. M.; FERREIRA, M. A.; LOUREIRO, M. E. Studies on proteins and enzymes related to tuverization and starch biosynthesis in cassava roots. Cali, Colombia: CIAT, 1993 234-238p CIAT, Working Document,123 International scientific meeting Cassava Biotechnology Network, 1, 1992, Cartajena, Colombia. Proceedinjs... Cali, Colombia: CIAT, 1993.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 7. | | LOUREIRO, M. E.; CADINELLI, G.; BARBOSA, W.; MATINEZ Y HUAMAN, C.; CARNEIRO, M. Physiological effects of the rolA gene expression in transgenic tobacco plants. In: ENCUENTRO LATINO AMERICANO DE BIOTECNOLOGIA VEGETAL, 2., 1995, Puerto Iguazu, Argentina. REDBIO95. [S.l.:s.n.], 1995. n.A-71.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 9. | | DIOLA, V.; BRITO, G. G. de; CAIXETA, E. T.; MACIEL-ZAMBOLIM, E.; SAKIYAMA, N. S.; LOUREIRO, M. E. High-density genetic mapping for coffee leaf rust resistance. Tree Genetics & Genomes, v. 7, n. 6, p. 1199-1208, Dec. 2011.| Biblioteca(s): Embrapa Café. |
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| 12. | | DIOLA, V.; LOUREIRO, M. E.; ALMEIDA, A. M. de; CAIXETA, E. T.; ZAMBOLIN, E. M.; BRITO, G. G. de. Expressão gênica em resposta a infecção de Hemileia vastatrix em café. In: CONGRESSO BRASILEIRO DE FISIOLOGIA VEGETAL, 12., 2009, Fortaleza. Desafios para produção de alimentos e bioenergia. Fortaleza: SBFV: UFC: Embrapa Agroindústria Tropical, 2009.| Biblioteca(s): Embrapa Algodão. |
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| 13. | | BRITO, G. G. de; CAIXETA, E. T.; GALLINA, A. P.; DIOLA, V.; ZAMBOLIM, E. M.; ZAMBOLIM, L.; LOUREIRO, M. E. Inheritance of coffee leaf rust resistance and identification of AFLP markers linked to the resistance gene. In: CONGRESSO BRASILEIRO DE FISIOLOGIA VEGETAL, 12., 2009, Fortaleza. Desafios para produção de alimentos e bioenergia. Fortaleza: SBFV: UFC: Embrapa Agroindústria Tropical, 2009.| Biblioteca(s): Embrapa Algodão. |
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| 15. | | BRITO, G. G. de; CAIXETA, E. T.; GALLINA, A. P.; ZAMBOLIM, E. M.; ZAMBOLIM, L.; DIOLA, V.; LOUREIRO, M. E. Inheritance of coffee leaf rust resistance and identification of AFLP markers linked to the resistance gene. Euphytica, Wageningen, v. 173, n. 2, p. 255-264, May 2010.| Biblioteca(s): Embrapa Algodão. |
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| 16. | | BRITO, G. G. D.; CAIXETA, E. T.; DIOLA, V.; ZAMBOLIM, E. M.; ZAMBOLIM, L.; LOUREIRO, M. E. Inheritance of coffee leaf rust resistance and identification of AFLP markers linked to the resistance gene. In:CONGRESSO BRASILEIRO DE FISIOLOGIA VEGETAL, 12., 2009, Fortaleza. Desafios para a produção de alimentos e bioenergia - resumos. Fortaleza: SBFV; UFC / EMBRAPA AGROINDÚSTIA TROPICAL, 2009.| Biblioteca(s): Embrapa Algodão. |
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| 17. | | RIBEIRO, M. C.; FIGUEIREDO, L. F. A.; LOUREIRO, M. E.; CABRAL, G. B.; CARVALHO, L. J. C. B. Study on the induction of storage root formation in cassava grown under in vitro culture and nutrition solution. In: THE CASSAVA biotechnology network: proceedings of the second international Scientific meeting, Bogor, Indonesia, 22-26 August 1994. Cali: CIAT, 1995. v.2, p.795-803. (CIAT. Working Document, n.150). v.2 p.795-803 (CIAT. Working Document, n.150)| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 18. | | DIOLA, V.; LOUREIRO, M. E.; BRITO, G. G. de; CAIXETA, E. T.; ZAMBOLIN, E. M.; SAKIYAMA, N. S.; PEREIRA, L. F. P. Construção do mapa genético e físico para um gene de resistência a Hemileia vastatrix em café. In: CONGRESSO BRASILEIRO DE FISIOLOGIA VEGETAL, 12., 2009, Fortaleza. Desafios para produção de alimentos e bioenergia. Fortaleza: SBFV: UFC: Embrapa Agroindústria Tropical, 2009.| Biblioteca(s): Embrapa Algodão. |
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| 19. | | DINIZ, L. E. C.; SAKIYAMA, N. S.; LASHERMES. P.; CAIXETA, E. T.; OLIVEIRA, A. C. B.; ZAMBOLIM, E. M.; LOUREIRO, M. E.; PEREIRA, A. A.; ZAMBOLIM, L. Analysis of AFLP markers associated to the Mex-1 resistance locus in Icatu progenies. Crop Breeding and Applied Biotechnology, Viçosa, MG, v. 5, n. 4, p. 387-393, Dec. 2005.| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 20. | | DIOLA, V.; CAÇÃO, S. M. B.; CAIXETA, E. T.; BRITO, G. G.; ZAMBOLIM, E. M.; PEREIRA, L. F. P.; LOUREIRO, M. E. Genetic and physical mapping of the resistant gene for H. vastatrix race II in Coffea arabica. In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 23. 2010, Bali, Indonesia.| Biblioteca(s): Embrapa Café. |
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| Registros recuperados : 42 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Algodão; Embrapa Café. |
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Data corrente: |
13/03/2014 |
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Data da última atualização: |
13/03/2014 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
DIOLA, V.; BRITO, G. G. de; CAIXETA, E. T.; PEREIRA, L. F. P.; LOUREIRO, M. E. |
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Afiliação: |
VALDIR DIOLA, UFV - VIÇOSA; GIOVANI GREIGH DE BRITO, CNPA; EVELINE TEIXEIRA CAIXETA, SAPC; LUIZ FILIPE PROTASIO PEREIRA, SAPC; MARCELO E. LOUREIRO, UFV - VIÇOSA. |
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Título: |
A new set of differentially expressed signaling genes is early expressed in coffee leaf rust race II incompatible interaction. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Functional & Integrative Genomics, v. 13, n. 3, p. 379-389, 2013. |
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Idioma: |
Inglês
Português |
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Conteúdo: |
New races of coffee rust are overcoming resistance genes available in germplasm and cultivated cultivars and bringing recently some coffee-producing countries in severe economic challenge. The objective of this study was to identify the genes that are linked to host resistance to the major coffee rust race II. In our study, we have identified and studied a segregating population that has a single monogenic resistant gene to coffee rust. Coffee leaves of parents, resistant, and susceptible genotypes of the F2 generation plants were inoculated with pathogen spores. A differential analysis was performed by combined cDNA-AFLP and bulk segregant analysis (BSA) in pooled samples collected 48 and 72 h postinoculation, increasing the selectiveness for differential gene expression. Of 108 differential expressed genes, between 33,000 gene fragments analyzed, 108 differential expressed genes were identified in resistant plants. About 20 and 22 % of these resistant-correlated genes are related to signaling and defense genes, respectively. Between signaling genes, the major subclass corresponds to receptor and resistant homolog genes, like nucleotide-binding site leucine-rich repeat (NBSLRR), Pto-like, RLKs, Bger, and RGH1A, all not previously described in coffee rust responses. The second major subclass included kinases, where two mitogen-activated kinases (MAPK) are identified. Further gene expression analysis was performed for 21 selected genes by real-time PCR gene expression analysis at 0, 12, 24, 48, and 72 h postinoculation. The expression of genes involved in signaling and defense was higher at 24 and 72 h after inoculation, respectively. The NBS-LRR was the more differentially expressed gene between the signaling genes (four times more expressed in the resistant genotype), and thraumatin (PR5) was the more expressed between all genes (six times more expressed). Multivariate analysis reinforces the significance of the temporal separation of identified signaling and defense genes: early expression of signaling genes support the hypothesis that higher expression of the signaling components up regulates the defense genes identified. Additionally the increased gene expression of these two gene sets is associated with a single monogenic resistance trait to to leaf coffee rust in the nteraction characterized here. MenosNew races of coffee rust are overcoming resistance genes available in germplasm and cultivated cultivars and bringing recently some coffee-producing countries in severe economic challenge. The objective of this study was to identify the genes that are linked to host resistance to the major coffee rust race II. In our study, we have identified and studied a segregating population that has a single monogenic resistant gene to coffee rust. Coffee leaves of parents, resistant, and susceptible genotypes of the F2 generation plants were inoculated with pathogen spores. A differential analysis was performed by combined cDNA-AFLP and bulk segregant analysis (BSA) in pooled samples collected 48 and 72 h postinoculation, increasing the selectiveness for differential gene expression. Of 108 differential expressed genes, between 33,000 gene fragments analyzed, 108 differential expressed genes were identified in resistant plants. About 20 and 22 % of these resistant-correlated genes are related to signaling and defense genes, respectively. Between signaling genes, the major subclass corresponds to receptor and resistant homolog genes, like nucleotide-binding site leucine-rich repeat (NBSLRR), Pto-like, RLKs, Bger, and RGH1A, all not previously described in coffee rust responses. The second major subclass included kinases, where two mitogen-activated kinases (MAPK) are identified. Further gene expression analysis was performed for 21 selected genes by real-time PCR gene expression analysi... Mostrar Tudo |
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Palavras-Chave: |
Defense response; Host-specific resistance; R-genes; Transcript-derived fragments. |
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Thesagro: |
Hemileia Vastatrix. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/99182/1/functional.pdf
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Marc: |
LEADER 03085naa a2200229 a 4500
001 1982262
005 2014-03-13
008 2013 bl uuuu u00u1 u #d
100 1 $aDIOLA, V.
245 $aA new set of differentially expressed signaling genes is early expressed in coffee leaf rust race II incompatible interaction.$h[electronic resource]
260 $c2013
520 $aNew races of coffee rust are overcoming resistance genes available in germplasm and cultivated cultivars and bringing recently some coffee-producing countries in severe economic challenge. The objective of this study was to identify the genes that are linked to host resistance to the major coffee rust race II. In our study, we have identified and studied a segregating population that has a single monogenic resistant gene to coffee rust. Coffee leaves of parents, resistant, and susceptible genotypes of the F2 generation plants were inoculated with pathogen spores. A differential analysis was performed by combined cDNA-AFLP and bulk segregant analysis (BSA) in pooled samples collected 48 and 72 h postinoculation, increasing the selectiveness for differential gene expression. Of 108 differential expressed genes, between 33,000 gene fragments analyzed, 108 differential expressed genes were identified in resistant plants. About 20 and 22 % of these resistant-correlated genes are related to signaling and defense genes, respectively. Between signaling genes, the major subclass corresponds to receptor and resistant homolog genes, like nucleotide-binding site leucine-rich repeat (NBSLRR), Pto-like, RLKs, Bger, and RGH1A, all not previously described in coffee rust responses. The second major subclass included kinases, where two mitogen-activated kinases (MAPK) are identified. Further gene expression analysis was performed for 21 selected genes by real-time PCR gene expression analysis at 0, 12, 24, 48, and 72 h postinoculation. The expression of genes involved in signaling and defense was higher at 24 and 72 h after inoculation, respectively. The NBS-LRR was the more differentially expressed gene between the signaling genes (four times more expressed in the resistant genotype), and thraumatin (PR5) was the more expressed between all genes (six times more expressed). Multivariate analysis reinforces the significance of the temporal separation of identified signaling and defense genes: early expression of signaling genes support the hypothesis that higher expression of the signaling components up regulates the defense genes identified. Additionally the increased gene expression of these two gene sets is associated with a single monogenic resistance trait to to leaf coffee rust in the nteraction characterized here.
650 $aHemileia Vastatrix
653 $aDefense response
653 $aHost-specific resistance
653 $aR-genes
653 $aTranscript-derived fragments
700 1 $aBRITO, G. G. de
700 1 $aCAIXETA, E. T.
700 1 $aPEREIRA, L. F. P.
700 1 $aLOUREIRO, M. E.
773 $tFunctional & Integrative Genomics$gv. 13, n. 3, p. 379-389, 2013.
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Embrapa Algodão (CNPA) |
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